Ukrainians currently living in the Citywest Hotel in Dublin have been notified that they must leave the accommodation by September.
The notice follows changes to the policy that granted temporary refuge to Ukrainians who fled the war, while Ukraine was considered unsafe.
Residents have been instructed to apply for accommodation in another State-supported centre. Applications, along with all required supporting documentation, must be submitted by 17 July.
Alternative accommodation is expected to be located across the country, with centres in counties including Louth, Offaly and Kildare.
Current Citywest residents are required to vacate the hotel by 18 September. Many believe the timeframe does not give them enough time to secure suitable private accommodation, particularly given Ireland's ongoing housing crisis.
In a statement, residents said they were grateful for the safety and opportunities Ireland has provided but believe the relocation process is not appropriate.
"Over the past four years, Ukrainians who fled Russia's war against Ukraine have rebuilt their lives in Ireland. They have found jobs, enrolled their children in schools, established medical care, and become part of their local communities. We are deeply grateful to Ireland for the safety and support it has provided to our families.
"Our protest is not against Ireland. It is a call for a fair, humane and individualised approach to relocation, ensuring that decisions take proper account of people’s health, disabilities, medical treatment, education, employment and other essential circumstances."
In a statement published on Facebook, Linda de Courcy said: "It is also hard on the children who have been living there since 2022, going to school and making friends, as well as the local children who have built friendships with the Ukrainian children."
Residents have also raised concerns that some of the proposed accommodation is unsuitable for families and vulnerable people, with reports that certain centres have shared toilets and shower facilities.
The Department of Justice said it is working with the Irish Red Cross and the International Organization for Migration to provide online information sessions in Ukrainian and Russian to help residents through the relocation process.
